Plasmid_Backbone

Part:BBa_K1137003:Design

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-10-04)


pSB9S1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1929
    Illegal suffix found at 3020
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1929
    Illegal NheI site found at 1005
    Illegal SpeI site found at 3021
    Illegal PstI site found at 3035
    Illegal NotI site found at 1935
    Illegal NotI site found at 3028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1929
    Illegal XhoI site found at 1805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1929
    Illegal suffix found at 3021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1929
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1944
    Illegal SpeI site found at 3021
    Illegal PstI site found at 3035
    Illegal NgoMIV site found at 258
    Illegal AgeI site found at 2731
    Illegal AgeI site found at 2843
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The terminators in the pSB1C3 made gibson assembly difficult and as such we switched to traditional cloning using XhoI and AvrII cut sites.


Source

Upgraded pSB1C3 swapping out the origin and resistance cassettes for CloDF13 and aadA from pCDF

References